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rabbit anti 53bp1  (Novus Biologicals)


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    Novus Biologicals rabbit anti 53bp1
    Rabbit Anti 53bp1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 854 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+53bp1+antibody/53BP1+Antibody+-+BSA+Free/pmc12919260-275-30-33
    Average 96 stars, based on 854 article reviews
    rabbit anti 53bp1 - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes
    Article Snippet: .. Then, neurons were incubated with the following buffers at RT, with two PBS washes after each incubation: primary antibody solution for 1 hour (Mouse Anti-phospho-Histone H2A.X Ser139 Antibody, clone JBW301, Sigma #05-636, 1:4000 diluted in blocking buffer; Rabbit Anti-53BP1 Antibody, Novus #100-305, 1:1000 diluted), secondary antibody solution for 1 hour (Goat antiMouse IgG H+L 568, Invitrogen #A-11031; Goat anti-Rabbit IgG H+L 488, Invitrogen #A-11034; both 1:1000 diluted in blocking buffer), then DAPI for 2 minutes (1:1000 diluted in PBS, Thermo #62248). ..

    Article Title: Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes
    Article Snippet: .. Then, neurons were incubated with the following buffers at RT, with two PBS washes after each incubation: primary antibody solution for 1 hour (Mouse Anti-phospho-Histone H2A.X Ser139 Antibody, clone JBW301, Sigma #05-636, 1:4000 diluted in blocking buffer; Rabbit Anti-53BP1 Antibody, Novus #100-305, 1:1000 diluted), secondary antibody solution for 1 hour (Goat anti-Mouse IgG H+L 568, Invitrogen #A-11031; Goat anti-Rabbit IgG H+L 488, Invitrogen #A-11034; both 1:1000 diluted in blocking buffer), then DAPI for 2 minutes (1:1000 diluted in PBS, Thermo #62248). ..

    Article Title: Characterizing and controlling CRISPR repair outcomes in nondividing human cells
    Article Snippet: .. Then, neurons were incubated with the following buffers at RT, with two PBS washes after each incubation: primary antibody solution for 1 h (Mouse Anti-phospho-Histone H2A.X Ser139 Antibody, clone JBW301, Sigma #05-636, 1:4000 diluted in blocking buffer; Rabbit Anti-53BP1 Antibody, Novus #100-305, 1:1000 diluted), secondary antibody solution for 1 h (Goat anti-Mouse IgG H + L 568, Invitrogen #A-11031; Goat anti-Rabbit IgG H + L 488, Invitrogen #A-11034; both 1:1000 diluted in blocking buffer), then DAPI for 2 min (1:1000 diluted in PBS, Thermo #62248). ..

    Blocking Assay:

    Article Title: Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes
    Article Snippet: .. Then, neurons were incubated with the following buffers at RT, with two PBS washes after each incubation: primary antibody solution for 1 hour (Mouse Anti-phospho-Histone H2A.X Ser139 Antibody, clone JBW301, Sigma #05-636, 1:4000 diluted in blocking buffer; Rabbit Anti-53BP1 Antibody, Novus #100-305, 1:1000 diluted), secondary antibody solution for 1 hour (Goat antiMouse IgG H+L 568, Invitrogen #A-11031; Goat anti-Rabbit IgG H+L 488, Invitrogen #A-11034; both 1:1000 diluted in blocking buffer), then DAPI for 2 minutes (1:1000 diluted in PBS, Thermo #62248). ..

    Article Title: Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes
    Article Snippet: .. Then, neurons were incubated with the following buffers at RT, with two PBS washes after each incubation: primary antibody solution for 1 hour (Mouse Anti-phospho-Histone H2A.X Ser139 Antibody, clone JBW301, Sigma #05-636, 1:4000 diluted in blocking buffer; Rabbit Anti-53BP1 Antibody, Novus #100-305, 1:1000 diluted), secondary antibody solution for 1 hour (Goat anti-Mouse IgG H+L 568, Invitrogen #A-11031; Goat anti-Rabbit IgG H+L 488, Invitrogen #A-11034; both 1:1000 diluted in blocking buffer), then DAPI for 2 minutes (1:1000 diluted in PBS, Thermo #62248). ..

    Article Title: Characterizing and controlling CRISPR repair outcomes in nondividing human cells
    Article Snippet: .. Then, neurons were incubated with the following buffers at RT, with two PBS washes after each incubation: primary antibody solution for 1 h (Mouse Anti-phospho-Histone H2A.X Ser139 Antibody, clone JBW301, Sigma #05-636, 1:4000 diluted in blocking buffer; Rabbit Anti-53BP1 Antibody, Novus #100-305, 1:1000 diluted), secondary antibody solution for 1 h (Goat anti-Mouse IgG H + L 568, Invitrogen #A-11031; Goat anti-Rabbit IgG H + L 488, Invitrogen #A-11034; both 1:1000 diluted in blocking buffer), then DAPI for 2 min (1:1000 diluted in PBS, Thermo #62248). ..

    Immunostaining:

    Article Title: MRE11 and TREX1 control senescence by coordinating replication stress and interferon signaling
    Article Snippet: .. Immunostaining was performed overnight at 4 °C with rabbit anti-53BP1 antibody (1:2000, Novus, NB100-304). ..

    Article Title: MRE11 and TREX1 control senescence by coordinating replication stress and interferon signaling
    Article Snippet: .. Immunostaining was performed overnight at 4°C with rabbit anti-53BP1 antibody (1:2000, Novus, NB100-304). ..

    Article Title: Regulation of oncogene-induced senescence by the MRE11 and TREX1 nucleases
    Article Snippet: .. Immunostaining was performed overnight at 4°C with rabbit anti-53BP1 antibody (1:2000, Novus, NB100-304). ..

    Immunofluorescence:

    Article Title: High-throughput telomere length measurement at nucleotide resolution using the PacBio high fidelity sequencing platform.
    Article Snippet: .. Nature Communications | (2023) 14:281 9 Immunofluorescence staining was carried out by incubating with a rabbit anti-53BP1 antibody (Novus Biologicals, NB100-304, 1:500) and a mouse anti-TRF1 antibody (Novus Biologicals, H00007013-B01, 1:500), followed by secondary antibodies Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (ThermoFisher Scientific, A-21202, 1:500) and Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 (ThermoFisher Scientific, A10042, 1:500) respectively. .. Cell images were acquired using a Nikon Ti-U microscope with a 100x objective and collected as a stack of 0.2μm increments in the z-axis.

    Staining:

    Article Title: High-throughput telomere length measurement at nucleotide resolution using the PacBio high fidelity sequencing platform.
    Article Snippet: .. Nature Communications | (2023) 14:281 9 Immunofluorescence staining was carried out by incubating with a rabbit anti-53BP1 antibody (Novus Biologicals, NB100-304, 1:500) and a mouse anti-TRF1 antibody (Novus Biologicals, H00007013-B01, 1:500), followed by secondary antibodies Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (ThermoFisher Scientific, A-21202, 1:500) and Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 (ThermoFisher Scientific, A10042, 1:500) respectively. .. Cell images were acquired using a Nikon Ti-U microscope with a 100x objective and collected as a stack of 0.2μm increments in the z-axis.



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    a Telomere length is characterized by MFI of TelC-FITC. b-d Summary data of the FCM for telomeric length of total, naive and memory CD4 T-cell frequencies in PBMCs from patients and HS. e Representative image and summary data of the FCM for the percentages of γ-H2AX level in CD4 T cells. f Representative image and summary data of WB analysis for the level of γ-H2AX in CD4 T cells. g Representative confocal microscopic images and summary data of co-localization of <t>53BP1</t> and γ-H2AX in nuclei (DNA damage foci).
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    Image Search Results


    a Telomere length is characterized by MFI of TelC-FITC. b-d Summary data of the FCM for telomeric length of total, naive and memory CD4 T-cell frequencies in PBMCs from patients and HS. e Representative image and summary data of the FCM for the percentages of γ-H2AX level in CD4 T cells. f Representative image and summary data of WB analysis for the level of γ-H2AX in CD4 T cells. g Representative confocal microscopic images and summary data of co-localization of 53BP1 and γ-H2AX in nuclei (DNA damage foci).

    Journal: bioRxiv

    Article Title: MRN-ATM Pathway Activation in CD4 T-Cell Senescence during Chronic Hepatitis B Virus Infection

    doi: 10.64898/2026.03.15.711849

    Figure Lengend Snippet: a Telomere length is characterized by MFI of TelC-FITC. b-d Summary data of the FCM for telomeric length of total, naive and memory CD4 T-cell frequencies in PBMCs from patients and HS. e Representative image and summary data of the FCM for the percentages of γ-H2AX level in CD4 T cells. f Representative image and summary data of WB analysis for the level of γ-H2AX in CD4 T cells. g Representative confocal microscopic images and summary data of co-localization of 53BP1 and γ-H2AX in nuclei (DNA damage foci).

    Article Snippet: The CD4 T cells were fixed in 2% paraformaldehyde for 20 minutes, permeabilized with 0.3% Triton X-100 in PBS for 10 minutes, blocked with 5% BSA in PBS for 1 hour, and then incubated with rabbit anti-53BP1 antibody (CST) and anti-γ-H2AX (Ser-139) antibody (Biolegend, San Diego, CA, USA) at 4 °C overnight.

    Techniques: